* 모든 제품은 부가세가 포함된 금액입니다.
＊재고 문의 T.031-723-7979 / E. info@cellto.co.kr / 카카오플러스 셀투바이오
Product Description
Exosomes, nano - sized vesicles released into extracellular environments such as body fluids (e.g., plasma, saliva, and urine), were initially considered cellular waste or by- products with limited significance in the field . However, recent discoveries have revealed their ability to carry a complex cargo of genetic materials, facilitating intercellular communication across different target tissues . This remarkable finding has sparked interest in exosomes as potential non- invasive diagnostic biomarkers and therapeutic nanocarriers . To further our understanding of their role as disease biomarkers and therapeutic agents, various methods for exosome isolation have been developed, leading to a growing number of related studies .
Achieving intact exosome isolation is a critical step in studying their biological functions . Traditional methods for extracellular vesicle (EV) isolation, such as ultracentrifugation, have been time - consuming, challenging, and prone to inconsistencies due to non- specific reagents . In response to these challenges, the XENO - EVI Plus Kit has been specifically designed to optimize the isolation of EVs from human plasma .
EV pellets isolated from plasma tend to aggregate easily, making resuspension difficult . However, XENO - EVI Plus Kit enhances suspension efficiency, thereby improving the accuracy of subsequent experiments .
▪ Time saving : no cumbersome ultracentrifugation step
▪ Cost - effective : more affordable than other costly kits
▪ Higher purity : reduces carry - over of non- exosomal proteins
▪ Easier resuspension of the EV pellet
General Guidelines
▪ The reaction size is based on using 100 ㎕ of plasma sample .
Product Performance
Figure 1. Extracellular vesicles (EVs) isolation and characterization. EVs were isolated from 100 μl of plasma using XENO-EVI and characterized by using ZetaView® nanoparticle tracking analysis (NTA) instrument (Particle Metrix).
Figure 2. Western blots for CD9 and albumin expression in exosome and plasma. Immunoblot analysis was performed to detect CD9 (EV marker protein) and albumin (highly abundant protein in plasma). Exosome in 100 μl of plasma was isolated using XENO-EVI Kit. Each lane was loaded with exosomal proteins and 500 ng of plasma total proteins. Western blot shows that exosome contains much more CD9 compared to plasma.
Figure 3. Analysis of exosome purity and the protein yield.
Exosomes were isolated from 100 μl of plasma using XENO-EVI and kits from company A and company B. Nanoparticle tracking analysis (NTA) was performed to count the number of particles by using a NanoSight NS300 system (Malvern Instruments). Total protein concentration was measured at 280 nm.
* 모든 제품은 부가세가 포함된 금액입니다.
＊재고 문의 T.031-723-7979 / E. info@cellto.co.kr / 카카오플러스 셀투바이오
Product Description
Exosomes, nano - sized vesicles released into extracellular environments such as body fluids (e.g., plasma, saliva, and urine), were initially considered cellular waste or by- products with limited significance in the field . However, recent discoveries have revealed their ability to carry a complex cargo of genetic materials, facilitating intercellular communication across different target tissues . This remarkable finding has sparked interest in exosomes as potential non- invasive diagnostic biomarkers and therapeutic nanocarriers . To further our understanding of their role as disease biomarkers and therapeutic agents, various methods for exosome isolation have been developed, leading to a growing number of related studies .
Achieving intact exosome isolation is a critical step in studying their biological functions . Traditional methods for extracellular vesicle (EV) isolation, such as ultracentrifugation, have been time - consuming, challenging, and prone to inconsistencies due to non- specific reagents . In response to these challenges, the XENO - EVI Plus Kit has been specifically designed to optimize the isolation of EVs from human plasma .
EV pellets isolated from plasma tend to aggregate easily, making resuspension difficult . However, XENO - EVI Plus Kit enhances suspension efficiency, thereby improving the accuracy of subsequent experiments .
▪ Time saving : no cumbersome ultracentrifugation step
▪ Cost - effective : more affordable than other costly kits
▪ Higher purity : reduces carry - over of non- exosomal proteins
▪ Easier resuspension of the EV pellet
General Guidelines
▪ The reaction size is based on using 100 ㎕ of plasma sample .
Product Performance
Figure 1. Extracellular vesicles (EVs) isolation and characterization. EVs were isolated from 100 μl of plasma using XENO-EVI and characterized by using ZetaView® nanoparticle tracking analysis (NTA) instrument (Particle Metrix).
Figure 2. Western blots for CD9 and albumin expression in exosome and plasma. Immunoblot analysis was performed to detect CD9 (EV marker protein) and albumin (highly abundant protein in plasma). Exosome in 100 μl of plasma was isolated using XENO-EVI Kit. Each lane was loaded with exosomal proteins and 500 ng of plasma total proteins. Western blot shows that exosome contains much more CD9 compared to plasma.
Figure 3. Analysis of exosome purity and the protein yield.
Exosomes were isolated from 100 μl of plasma using XENO-EVI and kits from company A and company B. Nanoparticle tracking analysis (NTA) was performed to count the number of particles by using a NanoSight NS300 system (Malvern Instruments). Total protein concentration was measured at 280 nm.
