XENO-EVIMEDI Kit
Extracellular Vesicle lsolation kit (from media)
Product Name : XENO-EVIMEDI™ KIT
Catalog Number : 9366-EVI-M
Product Description
Exosomes, nano-sized vesicles released into the extracellular environment such as body fluids (e.g., plasma, saliva, and urine), were initially regarded as cellular waste or byproducts with limited significance in the field. However, recent discoveries have unveiled their capacity to carry a complex cargo of genetic materials, facilitating intercellular communication among different target tissues. This remarkable finding has sparked interest in exosomes as potential non-invasive diagnostic biomarkers and therapeutic nanocarriers. To further our understanding of their role as diagnostic biomarkers of diseases and therapeutic applications, various approaches to exosome isolation have been developed, and the number of related studies is growing.
An intact isolation of exosomes is a critical step in studying the biological function of exosomes. Traditional methods for extracellular vesicle (EV) isolation, such as ultracentrifugation, have been time-consuming, challenging, and prone to inconsistencies due to non-specific reagents. In light of these challenges, the XENO-EVIMEDI™ KIT has been specifically designed to optimize the isolation of EVs from cell culture media.
▪ Time saving : no cumbersome ultracentrifugation step
▪ Cost-effective : more affordable than other costly kits
▪ Broadly applicable : covers various media types
General Guidelines
▪ The reaction size is based on using 500 μl of cell culture media sample.
▪ To prevent contamination of exosomes, use exosome depleted fetal bovine serum (FBS) instead of normal FBS as they contain unwanted exosomes.
▪ Related Product : XENO-EVI™ KIT, XENO-EVARI™ KIT
Product Performance
Figure 1. Extracellular vesicles (EVs) isolation using XENOEVIMEDI TM .The whole process of XENO-EVIMEDI TM takes around 3 hrs.
Without a cumbersome ultracentrifugation step, the XENO-EVIMEDI TM
method provides efficient, simple, and rapid outcomes to meet customers demands.
Figure 2. A and B. Exosomes were isolated from 1 ml of culture media using XENO-EVIMEDI TM and kits from company A and company B. Nanoparticle tracking analysis (NTA) was performed to count the number of particles by using a TWIN ZetaView (PMX-230) system (Particle Metrix). Red circles indicate unfavorable fractions after exosome isolation. C. TEM analyses of exosomes from two different types of neuronal cells. Size bar indicates 100 nm.
XENO-EVIMEDI Kit
Extracellular Vesicle lsolation kit (from media)
Product Name : XENO-EVIMEDI™ KIT
Catalog Number : 9366-EVI-M
Product Description
Exosomes, nano-sized vesicles released into the extracellular environment such as body fluids (e.g., plasma, saliva, and urine), were initially regarded as cellular waste or byproducts with limited significance in the field. However, recent discoveries have unveiled their capacity to carry a complex cargo of genetic materials, facilitating intercellular communication among different target tissues. This remarkable finding has sparked interest in exosomes as potential non-invasive diagnostic biomarkers and therapeutic nanocarriers. To further our understanding of their role as diagnostic biomarkers of diseases and therapeutic applications, various approaches to exosome isolation have been developed, and the number of related studies is growing.
An intact isolation of exosomes is a critical step in studying the biological function of exosomes. Traditional methods for extracellular vesicle (EV) isolation, such as ultracentrifugation, have been time-consuming, challenging, and prone to inconsistencies due to non-specific reagents. In light of these challenges, the XENO-EVIMEDI™ KIT has been specifically designed to optimize the isolation of EVs from cell culture media.
▪ Time saving : no cumbersome ultracentrifugation step
▪ Cost-effective : more affordable than other costly kits
▪ Broadly applicable : covers various media types
General Guidelines
▪ The reaction size is based on using 500 μl of cell culture media sample.
▪ To prevent contamination of exosomes, use exosome depleted fetal bovine serum (FBS) instead of normal FBS as they contain unwanted exosomes.
▪ Related Product : XENO-EVI™ KIT, XENO-EVARI™ KIT
Product Performance
Without a cumbersome ultracentrifugation step, the XENO-EVIMEDI TM
method provides efficient, simple, and rapid outcomes to meet customers demands.
Figure 2. A and B. Exosomes were isolated from 1 ml of culture media using XENO-EVIMEDI TM and kits from company A and company B. Nanoparticle tracking analysis (NTA) was performed to count the number of particles by using a TWIN ZetaView (PMX-230) system (Particle Metrix). Red circles indicate unfavorable fractions after exosome isolation. C. TEM analyses of exosomes from two different types of neuronal cells. Size bar indicates 100 nm.