* 모든 제품은 부가세가 포함된 금액입니다.
＊재고 문의 T.031-723-7979 / E. info@cellto.co.kr / 카카오플러스 셀투바이오
| Product Type | Monoclonal Antibody |
| Clone Number | AT4G6 |
| UniProt No. | P60709 |
| NCBI Accession No. | NP_001092 |
| Alternative names | Actin, cytoplasmic 1, Beta-actin |
| Host | Mouse |
| Reacts With | Human |
| Concentration | 0.2mg/ml (determined by BCA assay) |
| Formulation | Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol |
| Immunogen | Recombinant human ACTB (1-375aa) purified from E. coli |
| Isotype | IgG1 kappa |
| Purification | By protein-A affinity chromatography |
| Applications | ELISA, WB, ICC/IF |
| Usage | The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. |
| Storage | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Immunflorescense (ICC/IF)  | ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). |
 | The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: PC3 cell lysate Lane 4.: HepG2 cell lysate Lane 5.: MCF7 cell lysate Lane 6.: U87-MG cell lysate Lane 7.: NIH-3T3 cell lysate |
 | The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Kidney tissue lysate Lane 2.: Liver tissue lysate Lane 3.: Spleen tissue lysate Lane 4.: Bone marrow tissue lysate Lane 5.: Stomach tissue lysate |
 | The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ACTB antibody 1:1000 Lane 2.: ACTB antibody 1:3000 Lane 3.: ACTB antibody 1:5000 Lane 4.: ACTB antibody 1:10000 |
* 모든 제품은 부가세가 포함된 금액입니다.
＊재고 문의 T.031-723-7979 / E. info@cellto.co.kr / 카카오플러스 셀투바이오
| Product Type | Monoclonal Antibody |
| Clone Number | AT4G6 |
| UniProt No. | P60709 |
| NCBI Accession No. | NP_001092 |
| Alternative names | Actin, cytoplasmic 1, Beta-actin |
| Host | Mouse |
| Reacts With | Human |
| Concentration | 0.2mg/ml (determined by BCA assay) |
| Formulation | Liquid in. Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol |
| Immunogen | Recombinant human ACTB (1-375aa) purified from E. coli |
| Isotype | IgG1 kappa |
| Purification | By protein-A affinity chromatography |
| Applications | ELISA, WB, ICC/IF |
| Usage | The antibody has been tested by ELISA, Western blot and ICC/IF analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. |
| Storage | Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. |
Immunflorescense (ICC/IF) | ICC/IF analysis of ACTB in HeLa cells. The cell was stained with ATGA0570 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue). |
| The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: Jurkat cell lysate Lane 3.: PC3 cell lysate Lane 4.: HepG2 cell lysate Lane 5.: MCF7 cell lysate Lane 6.: U87-MG cell lysate Lane 7.: NIH-3T3 cell lysate |
| The mouse tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Kidney tissue lysate Lane 2.: Liver tissue lysate Lane 3.: Spleen tissue lysate Lane 4.: Bone marrow tissue lysate Lane 5.: Stomach tissue lysate |
| The HeLa cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human ACTB antibody. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: ACTB antibody 1:1000 Lane 2.: ACTB antibody 1:3000 Lane 3.: ACTB antibody 1:5000 Lane 4.: ACTB antibody 1:10000 |
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email : info@cellto.co.kr
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